Cell Fate Determination is especially targeted to grasp however a selected cell (or embryo) develops into the ultimate cell sort (or organism), basically, however, a cell's fate is decided. The development of recent molecular tools together with GFP and major advances in imaging technology as well as microscopy have created attainable the mapping of the cell lineage of Caenorhabditis elegans including its embryo. This technique of fate mapping is employed to review cells as they differentiate into their final cell fates. Merely perceptive a cell because it becomes differentiated throughout embryogenesis provides no indication of the mechanisms that drive the specification. Therefore, the addition of molecular manipulation techniques, as well as cistron and supermolecule knockdowns, knockouts and overexpression, together with living cell imaging techniques has been transformational in understanding what mechanisms are involved with cell fate determination.